Coding

Part:BBa_K1472610:Design

Designed by: Choi hiu Ying, LEE Cheuk Lam, Tse Yui Shing, Yeung Sin Yu   Group: iGEM14_CityU_HK   (2014-09-28)


Delta 15 desaturase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The enzyme cut site must be designed according to iGEM prefix and suffix requirements.


Source

The amino acid sequence of delta 15 desaturase is derived from Synechocystis sp. but codon optimised for the expression in E. coli.

References